Invitrogen

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

23 References

View all (8) IR/IGF1R antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Antibody in Western Blot (WB)

FIGURE: 1 / 12

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Antibody (44-804G) in WB

Upregulation and Antibody-Peptide Competition. Extracts of CHO-T cells over-expressing the human insulin receptor unstimulated (1) or stimulated with 100 nM insulin for 10 min at 37°C (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C and incubated with the IR/IGF1R (pYpY1162/1163) antibody for two hours at r... View More

Product Image Gallery

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of IR/IGF1R (pYpY1162/1163) was done on 70% confluent log phase MCF7 cells treated with insulin (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with IR/IGF1R (pYpY1162/1163) Rabbit polyclonal Antibody (Product # 44-804G) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing membrane localization. Panel e shows untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.

Phospho-IR/IGF1R (Tyr1162, Tyr1163) Antibody — Modulation of target protein phosphorylation by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of IR/IGF1R pY1162pY1163 using IR/IGF1R (pY1162pY1163) polyclonal antibody (Product # 44-804G) shows induced expression of IR/IGF1R (pY1162pY1163) in the membrane of MCF7 cells treated with insulin. {TM}

Product Details

44-804G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2533762

Product Specific Information

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody reactive with non-phosphorylated insulin/insulin-like growth factor-1 receptor (IR/IGF1R). The final product is generated by affinity chromatography using an IR/IGF1R-derived peptide that is phosphorylated at tyrosines 1162 and 1163 (tyrosines 1135 and 1136 for IGF1R. The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1150 and 1151 according to Ullrich et al.). The corresponding residues in the IGF1R are 1135 and 1136. The sequence is conserved in mouse and rat for both the IR and IGF1R. Although exhibiting a preference for IR/IGF1R, this antibody has been shown by both peptide competition and protein blotting to react with other dual-phosphotyrosine motifs from proteins such as c-Met and Shc.

This antibody has been used in western blotting and previous lots of this antibody have been used in immunostaining. Positive controls used with this antibody in western analysis were Chinese hamster ovary cells transfected with a vector containing human insulin receptor (CHO-T) and stimulated with insulin, or 3T3-L1 cells stimulated with insulin.

Target Information

Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma.

Cell

Chung KM,Singh J,Lawres L,Dorans KJ,Garcia C,Burkhardt DB,Robbins R,Bhutkar A,Cardone R,Zhao X,Babic A,Vayrynen SA,Dias Costa A,Nowak JA,Chang DT,Dunne RF,Hezel AF,Koong AC,Wilhelm JJ,Bellin MD,Nylander V,Gloyn AL,McCarthy MI,Kibbey RG,Krishnaswamy S,Wolpin BM,Jacks T,Fuchs CS,Muzumdar MD

44-804G was used in Immunohistochemistry to argue that PDAC progression is driven by local obesity-associated changes in the tumor microenvironment and implicate endocrine-exocrine signaling beyond insulin in PDAC development.

Thu May 14 00:00:00 EDT 2020

IGF2 Autocrine-Mediated IGF1R Activation Is a Clinically Relevant Mechanism of Osimertinib Resistance in Lung Cancer.

Molecular cancer research : MCR

Manabe T,Yasuda H,Terai H,Kagiwada H,Hamamoto J,Ebisudani T,Kobayashi K,Masuzawa K,Ikemura S,Kawada I,Hayashi Y,Fukui K,Horimoto K,Fukunaga K,Soejima K

44-804G was used in Immunohistochemistry to find that IGF2 autocrine-mediated IGF1R pathway activation is a clinically relevant and common mechanism of acquired resistance to osimertinib.

Wed Apr 01 00:00:00 EDT 2020

The inhibitory effect of ECG and EGCG dimeric procyanidins on colorectal cancer cells growth is associated with their actions at lipid rafts and the inhibition of the epidermal growth factor receptor signaling.

Biochemical pharmacology

Zhu W,Li MC,Wang FR,Mackenzie GG,Oteiza PI

44-804G was used in Western Blotting to shed light on a potential molecular mechanism on how procyanidins-rich diets may lower CRC risk.

Fri May 01 00:00:00 EDT 2020

Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism.

American journal of physiology. Endocrinology and metabolism

Nagarajan SR,Paul-Heng M,Krycer JR,Fazakerley DJ,Sharland AF,Hoy AJ

44-804G was used in Western Blotting to describe substrate metabolism of cultured AML12, IHH, and PH5CH8 cells, hepatocellular carcinoma-derived HepG2s, and primary mouse hepatocytes.

Mon Apr 01 00:00:00 EDT 2019

Ablation of Grb10 Specifically in Muscle Impacts Muscle Size and Glucose Metabolism in Mice.

Endocrinology

Holt LJ,Brandon AE,Small L,Suryana E,Preston E,Wilks D,Mokbel N,Coles CA,White JD,Turner N,Daly RJ,Cooney GJ

44-804G was used in Western Blotting to conclude that ablation of Grb10 in muscle is sufficient to affect muscle size and metabolism, supporting an important role for this protein in growth and metabolic pathways.

Thu Mar 01 00:00:00 EST 2018

Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase.

Nature chemical biology

Stanford SM,Aleshin AE,Zhang V,Ardecky RJ,Hedrick MP,Zou J,Ganji SR,Bliss MR,Yamamoto F,Bobkov AA,Kiselar J,Liu Y,Cadwell GW,Khare S,Yu J,Barquilla A,Chung TDY,Mustelin T,Schenk S,Bankston LA,Liddington RC,Pinkerton AB,Bottini N

Published figure using Phospho-IR/IGF1R (Tyr1162, Tyr1163) polyclonal antibody (Product # 44-804G) in Western Blot

Thu Jun 01 00:00:00 EDT 2017

Epigenetic regulation of macrophage polarization and inflammation by DNA methylation in obesity.

JCI insight

Wang X,Cao Q,Yu L,Shi H,Xue B,Shi H

44804G was used in western blot to show that DNA hypermethylation at the PPARgamma1 promoter is induced by obesity-related factors

Thu Nov 17 00:00:00 EST 2016

Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice.

Gastroenterology

Bettaieb A,Jiang JX,Sasaki Y,Chao TI,Kiss Z,Chen X,Tian J,Katsuyama M,Yabe-Nishimura C,Xi Y,Szyndralewiez C,Schröder K,Shah A,Brandes RP,Haj FG,Török NJ

44-804G was used in western blot to investigate the effects of nicotinamide adenine dinucleotide phosphate reduced oxidase 4 in liver tissues from patients with NASH and mice with steatohepatitis.

Sat Aug 01 00:00:00 EDT 2015

Agonistic aptamer to the insulin receptor leads to biased signaling and functional selectivity through allosteric modulation.

Nucleic acids research

Yunn NO,Koh A,Han S,Lim JH,Park S,Lee J,Kim E,Jang SK,Berggren PO,Ryu SH

44-804G was used in Western Blotting to investigate the potential of aptamers to the insulin receptor as target-specific agonists.

Fri Sep 18 00:00:00 EDT 2015

The insulin and IGF1 receptor kinase domains are functional dimers in the activated state.

Nature communications

Cabail MZ,Li S,Lemmon E,Bowen ME,Hubbard SR,Miller WT

Published figure using Phospho-IR/IGF1R (Tyr1162, Tyr1163) polyclonal antibody (Product # 44-804G) in Western Blot

Wed Mar 11 00:00:00 EDT 2015

Targeting the insulin-like growth factor receptor and Src signaling network for the treatment of non-small cell lung cancer.

Molecular cancer

Min HY,Yun HJ,Lee JS,Lee HJ,Cho J,Jang HJ,Park SH,Liu D,Oh SH,Lee JJ,Wistuba II,Lee HY

44804G was used in immunohistochemistry to study mechanisms that confer resistance to therapeutic interventions in the insulin-like growth factor receptor pathway

Thu Jun 04 00:00:00 EDT 2015

Methionine restriction restores a younger metabolic phenotype in adult mice with alterations in fibroblast growth factor 21.

Aging cell

Lees EK,Król E,Grant L,Shearer K,Wyse C,Moncur E,Bykowska AS,Mody N,Gettys TW,Delibegovic M

44-804G was used in western blot to examine the ability of methionine restriction to reverse age-induced obesity and insulin resistance in adult mice

Wed Oct 01 00:00:00 EDT 2014

Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity.

International journal of obesity (2005)

Pulinilkunnil T,Kienesberger PC,Nagendran J,Sharma N,Young ME,Dyck JR

44-804G was used in western blot to study the protective effects against cardiac steatosis and dilated cardiomyopathy of specifically overexpressing triglyceride lipase in cardiomyocytes in a murine model of diet-induced obesity.

Sat Feb 01 00:00:00 EST 2014

Fenretinide treatment prevents diet-induced obesity in association with major alterations in retinoid homeostatic gene expression in adipose, liver, and hypothalamus.

Diabetes

Mcilroy GD,Delibegovic M,Owen C,Stoney PN,Shearer KD,McCaffery PJ,Mody N

44-804G was used in western blot to test if alterations in retinoic acid-responsive genes contribute to the beneficial effects of fenretinide on obesity and insulin resistance.

Fri Mar 01 00:00:00 EST 2013

Early B-cell factor-1 (EBF1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.

The Journal of biological chemistry

Griffin MJ,Zhou Y,Kang S,Zhang X,Mikkelsen TS,Rosen ED

Published figure using Phospho-IR/IGF1R (Tyr1162, Tyr1163) polyclonal antibody (Product # 44-804G) in Western Blot

Fri Dec 13 00:00:00 EST 2013

Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wang HY,Bakshi K,Frankfurt M,Stucky A,Goberdhan M,Shah SM,Burns LH

Wed Jul 18 00:00:00 EDT 2012

Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.

The journals of gerontology. Series A, Biological sciences and medical sciences

Sequea DA,Sharma N,Arias EB,Cartee GD

44-804G was used in western blot to examine the insulin signaling pathway in isolated epitrochlearis and soleus muscles of old rats.

Sat Dec 01 00:00:00 EST 2012

Estrogen upregulates the IGF-1 signaling pathway in lung cancer through estrogen receptor-β.

Medical oncology (Northwood, London, England)

Tang H,Liao Y,Chen G,Xu L,Zhang C,Ju S,Zhou S

44-804G was used in immunohistochemistry - paraffin section to study estrogen regulated IGF-R signaling in non-small cell lung cancer.

Sat Dec 01 00:00:00 EST 2012

Conformation-sensing antibodies stabilize the oxidized form of PTP1B and inhibit its phosphatase activity.

Cell

Haque A,Andersen JN,Salmeen A,Barford D,Tonks NK

Fri Sep 30 00:00:00 EDT 2011

Knockdown of the Alström syndrome-associated gene Alms1 in 3T3-L1 preadipocytes impairs adipogenesis but has no effect on cell-autonomous insulin action.

International journal of obesity (2005)

Huang-Doran I,Semple RK

44-804G was used in western blot to knockdown Alms1 to investigate its role in adipocytes

Fri Oct 01 00:00:00 EDT 2010

BMS-536924 reverses IGF-IR-induced transformation of mammary epithelial cells and causes growth inhibition and polarization of MCF7 cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Litzenburger BC,Kim HJ,Kuiatse I,Carboni JM,Attar RM,Gottardis MM,Fairchild CR,Lee AV

Published figure using Phospho-IR/IGF1R (Tyr1162, Tyr1163) polyclonal antibody (Product # 44-804G) in Western Blot

Thu Jan 01 00:00:00 EST 2009

Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B.

The FEBS journal

Trümpler A,Schlott B,Herrlich P,Greer PA,Böhmer FD

Published figure using Phospho-IR/IGF1R (Tyr1162, Tyr1163) polyclonal antibody (Product # 44-804G) in Western Blot

Thu Oct 01 00:00:00 EDT 2009

Constitutively active type I insulin-like growth factor receptor causes transformation and xenograft growth of immortalized mammary epithelial cells and is accompanied by an epithelial-to-mesenchymal transition mediated by NF-kappaB and snail.

Molecular and cellular biology

Kim HJ,Litzenburger BC,Cui X,Delgado DA,Grabiner BC,Lin X,Lewis MT,Gottardis MM,Wong TW,Attar RM,Carboni JM,Lee AV

44-804G was used in Western Blotting to examine the transforming potential of type I insulin-like growth factor receptor in human mammary epithelial cells.

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; Insulin receptor subunit alpha; Insulin receptor subunit beta; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; IR; soluble IGF1R variant 1; soluble IGF1R variant 2

Gene Aliases: CD220; CD221; HHF5; IGF1R; IGFIR; IGFR; INSR; JTK13

UniProt ID: (Human) P08069, (Human) P06213

Entrez Gene ID: (Human) 3480, (Human) 3643

Molecular Function: transmembrane signal receptor