Immunofluorescence analysis of IR/IGF1R [pYpY1162/1163] was done on 70% confluent log phase MCF7 cells treated with insulin (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with IR/IGF1R [pYpY1162/1163] Rabbit polyclonal Antibody (44804G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing membrane localization. Panel e shows untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice.
44-804G was used in western blot to investigate the effects of nicotinamide adenine dinucleotide phosphate reduced oxidase 4 in liver tissues from patients with NASH and mice with steatohepatitis.
|Bettaieb A,Jiang JX,Sasaki Y,Chao TI,Kiss Z,Chen X,Tian J,Katsuyama M,Yabe-Nishimura C,Xi Y,Szyndralewiez C,Schröder K,Shah A,Brandes RP,Haj FG,Török NJ||Gastroenterology (149:468)||2015|
The insulin and IGF1 receptor kinase domains are functional dimers in the activated state.
44-804G was used in western blot to study the structures of the insulin receptor and the insulin-like growth factor-1 receptor
|Cabail MZ,Li S,Lemmon E,Bowen ME,Hubbard SR,Miller WT||Nature communications (6:null)||2015|
|Not Applicable||Not Cited||
Methionine restriction restores a younger metabolic phenotype in adult mice with alterations in fibroblast growth factor 21.
44-804G was used in western blot to examine the ability of methionine restriction to reverse age-induced obesity and insulin resistance in adult mice
|Lees EK,Król E,Grant L,Shearer K,Wyse C,Moncur E,Bykowska AS,Mody N,Gettys TW,Delibegovic M||Aging cell (13:817)||2014|
Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity.
44-804G was used in western blot to study the protective effects against cardiac steatosis and dilated cardiomyopathy of specifically overexpressing triglyceride lipase in cardiomyocytes in a murine model of diet-induced obesity.
|Pulinilkunnil T,Kienesberger PC,Nagendran J,Sharma N,Young ME,Dyck JR||International journal of obesity (2005) (38:205)||2014|
Early B-cell factor-1 (EBF1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.
44-804G was used in western blot to identify direct and indirect EBF targets in fat.
|Griffin MJ,Zhou Y,Kang S,Zhang X,Mikkelsen TS,Rosen ED||The Journal of biological chemistry (288:35925)||2013|
Fenretinide treatment prevents diet-induced obesity in association with major alterations in retinoid homeostatic gene expression in adipose, liver, and hypothalamus.
44-804G was used in western blot to test if alterations in retinoic acid-responsive genes contribute to the beneficial effects of fenretinide on obesity and insulin resistance.
|Mcilroy GD,Delibegovic M,Owen C,Stoney PN,Shearer KD,McCaffery PJ,Mody N||Diabetes (62:825)||2013|
Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.
44-804G was used in western blot to examine the insulin signaling pathway in isolated epitrochlearis and soleus muscles of old rats.
|Sequea DA,Sharma N,Arias EB,Cartee GD||The journals of gerontology. Series A, Biological sciences and medical sciences (67:1279)||2012|
|Mouse||Not Cited||Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.||Wang HY,Bakshi K,Frankfurt M,Stucky A,Goberdhan M,Shah SM,Burns LH||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:9773)||2012|
|Human||Not Cited||Conformation-sensing antibodies stabilize the oxidized form of PTP1B and inhibit its phosphatase activity.||Haque A,Andersen JN,Salmeen A,Barford D,Tonks NK||Cell (147:185)||2011|
|Not Applicable||Not Cited||
Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B.
44-804G was used in western blot to elucidate mechanisms of protein-tyrosine phosphatase degradation
|Trümpler A,Schlott B,Herrlich P,Greer PA,Böhmer FD||The FEBS journal (276:5622)||2009|
BMS-536924 reverses IGF-IR-induced transformation of mammary epithelial cells and causes growth inhibition and polarization of MCF7 cells.
44-804G was used in western blot to test a new insulin-like growth factor receptor tyrosine kinase inhibitor using MCF10A cells and human breast cancer cell lines
|Litzenburger BC,Kim HJ,Kuiatse I,Carboni JM,Attar RM,Gottardis MM,Fairchild CR,Lee AV||Clinical cancer research : an official journal of the American Association for Cancer Research (15:226)||2009|
Estrogen upregulates the IGF-1 signaling pathway in lung cancer through estrogen receptor-ß.
44-804G was used in immunohistochemistry - paraffin section to study estrogen regulated IGF-R signaling in non-small cell lung cancer.
|Tang H,Liao Y,Chen G,Xu L,Zhang C,Ju S,Zhou S||Medical oncology (Northwood, London, England) (29:2640)||2012|
Knockdown of the Alström syndrome-associated gene Alms1 in 3T3-L1 preadipocytes impairs adipogenesis but has no effect on cell-autonomous insulin action.
44-804G was used in western blot to knockdown Alms1 to investigate its role in adipocytes
|Huang-Doran I,Semple RK||International journal of obesity (2005) (34:1554)||2010|