Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of IR/IGF1R [pYpY1162/1163] was done on 70% confluent log phase MCF7 cells treated with insulin (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with IR/IGF1R [pYpY1162/1163] Rabbit polyclonal Antibody (44804G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing membrane localization. Panel e shows untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity.
44-804G was used in western blot to study the protective effects against cardiac steatosis and dilated cardiomyopathy of specifically overexpressing triglyceride lipase in cardiomyocytes in a murine model of diet-induced obesity.
|Pulinilkunnil T,Kienesberger PC,Nagendran J,Sharma N,Young ME,Dyck JR||International journal of obesity (2005) (38:205)||2014|
Early B-cell factor-1 (EBF1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.
44-804G was used in western blot to identify direct and indirect EBF targets in fat.
|Griffin MJ,Zhou Y,Kang S,Zhang X,Mikkelsen TS,Rosen ED||The Journal of biological chemistry (288:35925)||2013|
Fenretinide treatment prevents diet-induced obesity in association with major alterations in retinoid homeostatic gene expression in adipose, liver, and hypothalamus.
44-804G was used in western blot to test if alterations in retinoic acid-responsive genes contribute to the beneficial effects of fenretinide on obesity and insulin resistance.
|Mcilroy GD,Delibegovic M,Owen C,Stoney PN,Shearer KD,McCaffery PJ,Mody N||Diabetes (62:825)||2013|
Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.
||Wang HY,Bakshi K,Frankfurt M,Stucky A,Goberdhan M,Shah SM,Burns LH||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:9773)||2012|
Conformation-sensing antibodies stabilize the oxidized form of PTP1B and inhibit its phosphatase activity.
||Haque A,Andersen JN,Salmeen A,Barford D,Tonks NK||Cell (147:185)||2011|
Estrogen upregulates the IGF-1 signaling pathway in lung cancer through estrogen receptor-β.
44-804G was used in immunohistochemistry - paraffin section to study estrogen regulated IGF-R signaling in non-small cell lung cancer.
|Tang H,Liao Y,Chen G,Xu L,Zhang C,Ju S,Zhou S||Medical oncology (Northwood, London, England) (29:2640)||2012|
IGFIR, CD220, IGFR, CD221, HHF5, JTK13
IR, insulin receptor, IGF-I receptor, insulin-like growth factor 1 receptor, insulin-like growth factor I receptor, soluble IGF1R variant 1, soluble IGF1R variant 2, IGF1 Receptor, IGF-I Receptor, IGF1R, CD221, IGFIR, IGFR, JTK13, CD220, HHF5, INSR, Insulin receptor