|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein.[Swiss-Prot #O75460]|
|Purification||Antigen affinity chromatography|
|Contains||0.025% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with monkey samples.
Suggested positive control: Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells (20 ug).
IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease acitvities. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
LRP1 Protein Deficiency Exacerbates Palmitate-induced Steatosis and Toxicity in Hepatocytes.
PA1-16927 was used in western blot to analyze exacerbation of palmitate-induced steatosis and toxicity in hepatocytes by low density lipoprotein receptor-related protein-1 deficiency
|Hamlin AN,Basford JE,Jaeschke A,Hui DY||The Journal of biological chemistry (291:16610)||2016|
|Not Applicable||Not Cited||
HCV induces transforming growth factor ß1 through activation of endoplasmic reticulum stress and the unfolded protein response.
PA1-16927 was used in western blot to determine the induction of transforming growth factor beta1 through activation of endoplasmic reticulum stress and the unfolded protein response due to HCV
|Chusri P,Kumthip K,Hong J,Zhu C,Duan X,Jilg N,Fusco DN,Brisac C,Schaefer EA,Cai D,Peng LF,Maneekarn N,Lin W,Chung RT||Scientific reports (6:null)||2016|
Divergent androgen regulation of unfolded protein response pathways drives prostate cancer.
PA1-16927 was used in western blot to demonstrate that androgen receptor signaling modulates the unfolded protein response in prostate cancer cells.
|Sheng X,Arnoldussen YJ,Storm M,Tesikova M,Nenseth HZ,Zhao S,Fazli L,Rennie P,Risberg B,Wæhre H,Danielsen H,Mills IG,Jin Y,Hotamisligil G,Saatcioglu F||EMBO molecular medicine (7:788)||2015|
CD133¿ melanoma subpopulation acquired resistance to caffeic acid phenethyl ester-induced apoptosis is attributed to the elevated expression of ABCB5: significance for melanoma treatment.
PA1-16927 was used in western blot to study the effect of caffeic acid phenethyl ester on CD133+ melanoma
|El-Khattouti A,Sheehan NT,Monico J,Drummond HA,Haikel Y,Brodell RT,Megahed M,Hassan M||Cancer letters (357:83)||2015|
|Not Applicable||Not Cited||
Methionine restriction restores a younger metabolic phenotype in adult mice with alterations in fibroblast growth factor 21.
PA1-16927 was used in western blot to examine the ability of methionine restriction to reverse age-induced obesity and insulin resistance in adult mice
|Lees EK,Król E,Grant L,Shearer K,Wyse C,Moncur E,Bykowska AS,Mody N,Gettys TW,Delibegovic M||Aging cell (13:817)||2014|
Dipeptidyl petidase-IV inhibitor (gemigliptin) inhibits tunicamycin-induced endoplasmic reticulum stress, apoptosis and inflammation in H9c2 cardiomyocytes.
PA1-16927 was used in western blot to study the ability of pharmacological inhibition of DPP IV to protect cardiomyocytes against apoptosis and inflammation caused by tunicamycin-induced ER stress
|Hwang HJ,Jung TW,Ryu JY,Hong HC,Choi HY,Seo JA,Kim SG,Kim NH,Choi KM,Choi DS,Baik SH,Yoo HJ||Molecular and cellular endocrinology (392:1)||2014|
Inhibition of JNK by novel curcumin analog C66 prevents diabetic cardiomyopathy with a preservation of cardiac metallothionein expression.
PA1-16927 was used in western blot to study the role of cardiac metallothionein expression maintenance in the mechanisms by which inhibition of JNK by a curcumin analog protects against cardiomyopathy in a rat model of diabetes
|Wang Y,Zhou S,Sun W,McClung K,Pan Y,Liang G,Tan Y,Zhao Y,Liu Q,Sun J,Cai L||American journal of physiology. Endocrinology and metabolism (306:E1239)||2014|
Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells.
PA1-16927 was used in western blot to study the ability of mReg2-induced expression of GPR78 to protect murine insulinoma cells against endoplasmic reticulum stress
|Liu L,Chowdhury S,Fang X,Liu JL,Srikant CB||FEBS letters (588:2016)||2014|
Unfolded protein response signaling by transcription factor XBP-1 regulates ADAM10 and is affected in Alzheimer's disease.
PA1-16927 was used in western blot to study the regulation of ADAM10 expression by the unfolded protein response transcription factor XBP-1 and the implications for Alzheimer's disease
|Reinhardt S,Schuck F,Grösgen S,Riemenschneider M,Hartmann T,Postina R,Grimm M,Endres K||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (28:978)||2014|
CYP2J2-derived epoxyeicosatrienoic acids suppress endoplasmic reticulum stress in heart failure.
PA1-16927 was used in western blot to study the suppression of ER stress in the failing heart by epoxyeicosatrienoic acids generated from arachidonic acid by CYP2J2
|Wang X,Ni L,Yang L,Duan Q,Chen C,Edin ML,Zeldin DC,Wang DW||Molecular pharmacology (85:105)||2014|
Vinblastine-induced apoptosis of melanoma cells is mediated by Ras homologous A protein (Rho A) via mitochondrial and non-mitochondrial-dependent mechanisms.
PA1-16927 was used in western blot to study the role of Rho A-mediated mitochondrial and non-mitochondrial pathways in the induction of melanoma cell apoptosis by vinblastine
|Selimovic D,Badura HE,El-Khattouti A,Soell M,Porzig BB,Spernger A,Ghanjati F,Santourlidis S,Haikel Y,Hassan M||Apoptosis : an international journal on programmed cell death (18:980)||2013|
Monitoring endoplasmic reticulum stress responsive mRNAs by RNA sequencing.
PA1-16927 was used in western blot to identify endoplasmic reticulum stress responsive mRNAs by RNA sequencing.
|Okuda T,Wakaguri H,Suzuki Y,Sugano S||Gene (500:63)||2012|
ER stress negatively modulates the expression of the miR-199a/214 cluster to regulates tumor survival and progression in human hepatocellular cancer.
PA1-16927 was used in western blot to study the role of ER stress in the downregulation of the miRNA-199a/214 cluster observed in human hepaticellular cancer
|Duan Q,Wang X,Gong W,Ni L,Chen C,He X,Chen F,Yang L,Wang P,Wang DW||PloS one (7:null)||2012|
Apoptosis-related protein-2 triggers melanoma cell death by a mechanism including both endoplasmic reticulum stress and mitochondrial dysregulation.
PA1-16927 was used in western blot to investigate the mechanism for the cell apoptosis stimulated by APR 2 in cancer cells
|Selimovic D,Ahmad M,El-Khattouti A,Hannig M,Haïkel Y,Hassan M||Carcinogenesis (32:1268)||2011|
Constitutive role for IRE1¿-XBP1 signaling pathway in the insulin-mediated hepatic lipogenic program.
PA1-16927 was used in western blot to study the involvement of IRE1-XBP1 signaling pathway in hepatic lipogenesis
|Ning J,Hong T,Ward A,Pi J,Liu Z,Liu HY,Cao W||Endocrinology (152:2247)||2011|
HDLs inhibit endoplasmic reticulum stress and autophagic response induced by oxidized LDLs.
PA1-16927 was used in western blot to investigate the inhibitory effect of HDLs on the endoplasmic reticulum stress and autophagic response induced by oxidized LDLs
|Muller C,Salvayre R,Nègre-Salvayre A,Vindis C||Cell death and differentiation (18:817)||2011|
Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3.
PA1-16927 was used in western blot to study the mechanism for the resistance against adenoviral REIC/Dkk-3 gene therapy in a subpopulation of prostate cancer cells
|Tanimoto R,Sakaguchi M,Abarzua F,Kataoka K,Kurose K,Murata H,Nasu Y,Kumon H,Huh NH||International journal of cancer (126:1562)||2010|
Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-¿B inhibition.
PA1-16927 was used in western blot to report the mechanism by which imiquimod induces apoptosis in melanoma cells
|El-Khattouti A,Selimovic D,Hannig M,Taylor EB,Abd Elmageed ZY,Hassan SY,Haikel Y,Kandil E,Leverkus M,Brodell RT,Megahed M,Hassan M||Journal of cellular and molecular medicine (20:266)||2016|
Attenuation of oxygen fluctuation-induced endoplasmic reticulum stress in human lens epithelial cells.
PA1-16927 was used in western blot to study oxygen-induced endoplasmic reticulum stress in the lens
|Zheng XY,Xu J,Chen XI,Li W,Wang TY||Experimental and therapeutic medicine (10:1883)||2015|
TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling.
PA1-16927 was used in western blot to study the role of transmembrane protein 33 in the unfolded protein response
|Sakabe I,Hu R,Jin L,Clarke R,Kasid UN||Breast cancer research and treatment (153:285)||2015|
PARP16 is a tail-anchored endoplasmic reticulum protein required for the PERK- and IRE1¿-mediated unfolded protein response.
PA1-16927 was used in immunocytochemistry and immunoprecipitation to study the unfolded protein response mediated by PERK and IRE1-alpha and the key role of PARP16
|Jwa M,Chang P||Nature cell biology (14:1223)||2012|