This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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PA1-1054 detects phosphorylated IRS-1 (Ser307) in mouse and human cells.
PA1-1054 has been successfully used in Western Blot procedures. By WB, this antibody detects an ~170 kDa protein representing phospho-IRS-1 (Ser307) from 3T3 L1 cells treated with insulin for 10 minutes.
The PA1-1054 immunogen is a synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1. This peptide is 100% conserved in rats and mice. This peptide (Cat. #PEP-184) is available for use in neutralization and control experiments.
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1