PA1-1060 detects phosphorylated IRS-1 (Tyr1173) in mouse and human cells.
PA1-1060 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Tyr1173) from 3T3 L1 cells treated with insulin.
The PA1-1060 immunogen is a synthetic phosphopeptide corresponding to residues L(1173) E N G L N (pY) I D L D L V K(1186) of human IRS-1. This immunogenic sequence is 100% conserved in humans and chickens and 85% and 87% conserved in rat and mouse, respectively. The conserved phosphorylation site corresponds to residue Y1179 in human, Y1177 in chicken, Y1173 in mouse, and Y1172 in rat. This peptide (Cat. # PEP-205) is available for use in neutralization and control experiments.
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic target.
To date there have been four subtypes identified: IRS-1, 2, 3 and 4, with IRS-1 being widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; IRS-1; Phospho-Insulin Receptor Substrate-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1