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|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Hamster , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IRS-1 that contains serine 307 (serine 302 in mouse sequence).|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm"e;s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reduction of JNK1 expression with antisense oligonucleotide improves adiposity in obese mice.
||Yu XX,Murray SF,Watts L,Booten SL,Tokorcheck J,Monia BP,Bhanot S||American journal of physiology. Endocrinology and metabolism (295:E436)||2008|
Serine phosphorylation proximal to its phosphotyrosine binding domain inhibits insulin receptor substrate 1 function and promotes insulin resistance.
||Liu YF,Herschkovitz A,Boura-Halfon S,Ronen D,Paz K,Leroith D,Zick Y||Molecular and cellular biology (24:9668)||2004|
HIRS-1, IRS1IRM, IRS-1, G972R
IRS-1, insulin receptor substrate 1, pp185, IRS1, Insulin receptor substrate-1