|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corrsponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-1055 detects phospho-IRS-1 (Ser636) in mouse and human cells.
PA1-1055 has been successfully used in WB procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Ser636) from 3T3 L1 cells treated with insulin for 10 minutes.
The PA1-1055 immunogen is a synthetic phosphopeptide corrsponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1. The peptide sequence is 100% conserved in rats, and mice. This peptide (Cat. # PEP-185) is available for use in neutralization and control experiments.
Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm and quote;s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option.To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.
PA1-1055 was used in western blot to study the important role for ROS production in adipocytes and the associated insulin resistance and inflammatory response
|Lin Y,Berg AH,Iyengar P,Lam TK,Giacca A,Combs TP,Rajala MW,Du X,Rollman B,Li W,Hawkins M,Barzilai N,Rhodes CJ,Fantus IG,Brownlee M,Scherer PE||The Journal of biological chemistry (280:4617)||2005|