Western blot analysis of Phospho-Interferon gamma Receptor 1 pTyr457 using Phospho-Interferon gamma Receptor 1 pTyr457 polyclonal antibody (Product # PA5-36812) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with IFN-gamma (100ug/ml, 15min), Lane 2: NIH-3T3 cell lysate treated with IFN-gamma (100ug/ml, 15min), Lane 3: H9C2 cell lysate treated with IFN-gamma (100ug/ml, 15min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human IFN- gammaR alpha around the phosphorylation site of Tyrosine 457|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 54 and 90 kDa.
Purity is >95% by SDS-PAGE.
IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.