|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human JAK3 around the phosphorylation site of Tyr785 (S-D-YP-E-L)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
JAK 3 (also designated L JAK) is a member of the Janus family of protein tyrosine kinases along with JAK 1, JAK 2, or Tyk 2. Subsequent to activation, these kinases function to activate various members of the Stat family of transcription factors by phosphorylation on critical tyrosine regulatory sites. JAK 3 is activated in response to IL-3 and IL-4 in T cells and myeloid cells. JAK 3 activation requires the serine rich membrane proximal region of the IL 2 receptor beta chain, but does not require the acidic domain that is required for association and activation of Src family kinases. Expression of JAK 3 is restricted to NK and T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.