|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphorylated peptide (pT183-P-pY185) based on human JNK|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:500 - 1:2000|
|Immunohistochemistry (IHC)||1:500 - 1:2000|
|Western Blot (WB)||1:2000 -1:4000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9593 detects Phospho-JNK/SAPK in human, mouse, chicken and rat samples.
PA1-9593 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures. By Western blot, PA1-9593 detects a 46 kDa and 54 kDa protein representing Phospho-JNK/SAPK.
PA1-9593 immunogen is a phosphorylated peptide (pT183-P-pY185) based on human JNK.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.