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      View (8) other JNK1/JNK2 antibodies

      Invitrogen

      Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (D12H7L17), ABfinity™ Rabbit Monoclonal

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      Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated. View details.

      References Icon 4 References
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      Catalog #:700031

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      Product Details

      TESTED APPLICATIONS DILUTION
      ChIP assay (ChIP) 1 ul
      ELISA (ELISA) 1-5 ug/ml
      Flow Cytometry (Flow) 0.5-5 µg/10^6 cells
      Immunocytochemistry (ICC) 4-6 ug/ml
      Immunofluorescence (IF) 4-6 ug/ml
      Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:100
      Western Blot (WB) 0.1-1 ug/ml
      PUBLISHED APPLICATIONS
      Western Blot (WB) See 4 publications below
      Tested Species reactivity Human, Mouse
      Published species reactivity Insect , Mouse , Human
      Host / Isotype Rabbit / IgG
      Class Monoclonal
      Type Antibody
      Clone D12H7L17
      Immunogen A peptide corresponding to amino acids 179-189 and 180-189 of P45983 and P45984, respectively.
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/ml
      Purification Protein A
      Storage buffer PBS
      Contains 0.09% sodium azide
      Storage conditions Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
      RRID AB_2532273

      Product Specific Information

      This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.

      ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.

      ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.

      Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

      Background/Target Information

      C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (4)

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      Bioinformatics

      Protein Aliases: c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; JNK; JNK-46; JNK-55; JNK/SAPK alpha; JNK1; JNK1 beta1 protein kinase; JNK1A2; JNK2; JNK21B1/2; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK 8; MAPK 9; MAPK8; MAPK9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; Phospho-JNK; PRKM8; PRKM9; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK1; SAPK1A; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2

      Gene Aliases: AI849689; AI851083; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK8; MAPK9; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1A; SAPK1C

      UniProt ID: (Human) P45983, (Human) P45984, (Mouse) Q91Y86, (Mouse) Q9WTU6

      Entrez Gene ID: (Human) 5599, (Human) 5601, (Mouse) 26419, (Mouse) 26420

      Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase

      Function(s) Blue Arrow Icon
      protein phosphorylation response to stress JNK cascade JUN phosphorylation response to UV positive regulation of gene expression regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of histone deacetylation positive regulation of cyclase activity negative regulation of protein binding regulation of protein localization Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process rhythmic process regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein metabolic process cellular response to lipopolysaccharide cellular response to mechanical stimulus positive regulation of deacetylase activity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway release of cytochrome c from mitochondria positive regulation of protein phosphorylation protein targeting to mitochondrion activation of cysteine-type endopeptidase activity involved in apoptotic process central nervous system development response to mechanical stimulus response to toxic substance positive regulation of macrophage derived foam cell differentiation positive regulation of cell morphogenesis involved in differentiation response to amine neuron projection development positive regulation of prostaglandin biosynthetic process regulation of protein ubiquitination positive regulation of prostaglandin secretion positive regulation of chemokine production cellular response to UV response to drug positive regulation of nitric oxide biosynthetic process positive regulation of transcription, DNA-templated regulation of JNK cascade response to cadmium ion positive regulation of nitric-oxide synthase biosynthetic process cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to growth factor stimulus positive regulation of podosome assembly positive regulation of apoptotic signaling pathway ossification regulation of transcription, DNA-templated inflammatory response response to osmotic stress signal transduction response to heat regulation of gene expression phosphorylation positive regulation of cell migration positive regulation of microtubule polymerization response to hydrogen peroxide positive regulation of DNA replication determination of dorsal identity cellular response to hydrogen peroxide cellular response to nitric oxide type B pancreatic cell apoptotic process neuronal stem cell population maintenance programmed necrotic cell death positive regulation of determination of dorsal identity positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
      Process(es) Blue Arrow Icon
      protein serine/threonine kinase activity
       JUN kinase activity
       protein binding
       ATP binding
       kinase activity
       enzyme binding
       histone deacetylase regulator activity
       histone deacetylase binding
       transcription factor binding
       cysteine-type endopeptidase activator activity involved in apoptotic process
       mitogen-activated protein kinase kinase kinase binding
       nucleotide binding
       protein kinase activity
       MAP kinase activity
       transferase activity
       transferase activity, transferring phosphorus-containing groups

      Suggested Secondary Antibodies

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