Immunofluorescent analysis of Phospho-JNK1/JNK2 pThr183+pTyr185 in HeLa cells using a Phospho-JNK1/JNK2 pThr183+pTyr185 recombinant rabbit monoclonal antibody (Product # 700031) at a dilution of 5ug/ml in the absence of peptide (left) or in the presence of the immunogenic peptide (right), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Insect, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 179-189 and 180-189 of P45983 and P45984, respectively.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 ul|
|ELISA (ELISA)||1-5 ug/ml|
|Flow Cytometry (Flow)||0.5-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||4-6 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor necrosis factor-alpha mediates activation of NF-¿B and JNK signaling cascades in retinal ganglion cells and astrocytes in opposite ways.
700031 was used in western blot to study the activation of NF-kappaB and JNK signaling cascades mediated by tumor necrosis factor-alpha activation in retinal ganglion cells and astrocytes in opposite ways
|Dvoriantchikova G,Ivanov D||The European journal of neuroscience (40:3171)||2014|
Purinergic P2Y¿ receptors promote neutrophil infiltration and hepatocyte death in mice with acute liver injury.
700031 was used in western blot to study the role of inflammation and purinergic P2 receptors in acute hepatitis.
|Ayata CK,Ganal SC,Hockenjos B,Willim K,Vieira RP,Grimm M,Robaye B,Boeynaems JM,Di Virgilio F,Pellegatti P,Diefenbach A,Idzko M,Hasselblatt P||Gastroenterology (143:1620)||2012|
The transcription factor c-Jun protects against sustained hepatic endoplasmic reticulum stress thereby promoting hepatocyte survival.
700031 was used in western blot to investigate the protective effect of c-Jun against hepatic endoplasmic reticulum stress.
|Fuest M,Willim K,MacNelly S,Fellner N,Resch GP,Blum HE,Hasselblatt P||Hepatology (Baltimore, Md.) (55:408)||2012|
|Insect||Not Cited||The mitogen-activated protein kinome from Anopheles gambiae: identification, phylogeny and functional characterization of the ERK, JNK and p38 MAP kinases.||Horton AA,Wang B,Camp L,Price MS,Arshi A,Nagy M,Nadler SA,Faeder JR,Luckhart S||BMC genomics (12:null)||2011|