Western blot analysis of Phospho-JNK1 + JNK2 pThr183 + pTyr185 in 293, untreated or treated with UV, and PC12 cells untreated or treated with sorbitol. Samples were probed with a Phospho-JNK1 + JNK2 pThr183 + pTyr185 polyclonal antibody (Product # PA1-28425).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28425 detects JNK1 + JNK2 phospho T183, Y185 from human samples. PA1-28425 is expected to react with a wide range of other species due to sequence homology.
PA1-28425 has been successfully used in Western blot applications.
The PA1-28425 immunogen is a synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence. This region is conserved among many species including human, rat, mouse, chick, nematode
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. The jun family member, cJun, is phosphorylated by another kinase termed cJun N-terminal kinase (JNK).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.