Immunofluorescent analysis of SY5Y cells using a Phospho-KLF4 pSer245 polyclonal antibody (Product # PA5-13081). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with a Phospho-KLF4 pSer245 polyclonal antibody (Product # PA5-13081) (1:500, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Cytoplasmic actin was counterstained with red dye conjugated phalloidin (5.25 uM, 25 min).
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S245 of human KLF4|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with mouse based on sequence homology.
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Post-Transcriptional Regulation of KLF4 by High-Risk Human Papillomaviruses Is Necessary for the Differentiation-Dependent Viral Life Cycle.
PA5-13081 was used in immunocytochemistry and western blot to determine the necessity for differentiation-dependent viral life cycle by post-transciptional regulation of KLF4 by high-risk human papillomaviruses
|Gunasekharan VK,Li Y,Andrade J,Laimins LA||PLoS pathogens (12:null)||2016|