|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human LAT that contains tyrosine 132.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.
44-224 was used in western blot to examine the quantitative and temporal requirements of Zap70's function in thymocyte development
|Au-Yeung BB,Melichar HJ,Ross JO,Cheng DA,Zikherman J,Shokat KM,Robey EA,Weiss A||Nature immunology (15:687)||2014|
|Human||Not Cited||Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response.||Deindl S,Kadlecek TA,Cao X,Kuriyan J,Weiss A||Proceedings of the National Academy of Sciences of the United States of America (106:20699)||2009|
|Human||Not Cited||Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.||Brdicka T,Kadlecek TA,Roose JP,Pastuszak AW,Weiss A||Molecular and cellular biology (25:4924)||2005|