Western blot detection of Phospho-LAT pTyr132 in extracts prepared from Jurkat E6.1 cells were left unstimulated (lane 1) or stimulated (lanes 2-5). Samples were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C and probed using a Phospho-LAT pTyr132 polyclonal antibody (Product # PA1-26640).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human LAT containing tyrosine 132.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26640 detects LAT (phospho Y132) from human samples. PA1-26640 is expected to cross react with mouse (85% conserved), rat (77% conserved) due to sequence homology.
PA1-26640 has been successfully used in Western blot procedures.
The PA1-26640 immunogen is a synthetic phosphopeptide corresponding to a portion of human LAT containing tyrosine 132.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.