Background extracts alone (1) or with added recombinant active human Lck protein (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with a Phospho-Lck (pTyr192) antibody (Product # PA1-26702) in a 1% BSA-TBST buffer for two hours at room temperature, following incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4) or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugated secondary antibody. The data show that only the phosphopeptide corresponding to Lck (pTrY192) blocks the antibody signal, demonstrating the specificity of the antibody.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human Lck containing tyrosine 192. The sequence is conserved in human and mouse.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26702 detects Lck (phospho Y192) from human samples. PA1-26702 is expected to cross react with chicken (79% conserved) and mouse (100% conserved) due to sequence homology.
PA1-26702 has been successfully used in Western blot procedures.
The PA1-26702 immunogen is a synthetic phosphopeptide corresponding to a portion of human Lck containing tyrosine 192. The sequence is conserved in human and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.