Invitrogen

Phospho-LIMK1 (Thr508) Polyclonal Antibody

View all (59) LIMK1 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

Phospho-LIMK1 (Thr508) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-LIMK1 (Thr508) in NIH-3T3. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-LIMK1 (Thr508) polyclonal antibody (Product # PA5-104925) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Phospho-LIMK1 (Thr508) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-LIMK1 (Thr508) in human renal clear cell carcinoma tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-LIMK1 (Thr508) polyclonal antibody (Product # PA5-104925) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-LIMK1 (Thr508) Antibody in Immunohistochemistry (IHC) — Immunofluorescent analysis of Phospho-LIMK1 (Thr508) in human gastric tissue sections. Samples were incubated with Phospho-LIMK1 (Thr508) polyclonal antibody (Product # PA5-104925) using a dilution of 1:200.

Phospho-LIMK1 (Thr508) Antibody in Western Blot (WB) — Western blot analysis of Phospho-LIMK1 (Thr508) in HeLa cells (Lane 1: Hela cell extract; Lane 2: Hela and H2O2 cell extract; Lane 3: p-peptide Rat brain tissue). Samples were incubated with Phospho-LIMK1 (Thr508) polyclonal antibody (Product # PA5-104925).

Product Details

PA5-104925

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

RRID

AB_2816398

Target Information

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.11.1; kinase LIMK1; KIZ-1; LIM domain kinase 1; lim kinase 1; LIM motif-containing protein kinase; LIM motif-containing protein kinase 1; LIM-domain containing protein kinase 1; LIM-domain containing, protein kinase 1; LIMK-1

Gene Aliases: LIMK; LIMK1