|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human LIMK1/2 around the phosphorylation site of Thr508/505|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.