Western blot analysis of Phospho-LKB1 pSer428 using Phospho-LKB1 pSer428 polyclonal antibody (Product # PA5-36858) at a dilution of 1:500. Lane 1: Hela cell lysate treated with UV (24h), Lane 2: NIH-3T3 cell lysate treated with UV (24h), Lane 3: PC12 cell lysate treated with UV (24h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Serine 428|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 54 kDa.
Purity is >95% by SDS-PAGE.
LKB1, a member of the serine/threonine kinase family, is a tumor suppressor. Physically associates with p53, it regulates specific p53 dependent apoptosis pathways. LKB1 protein is present in both the cytoplasm and nucleus of living cells and translocates to mitochondria during apoptosis. Restoring LKB1 activity into cancer cell lines defective for its expression results in a G1 cell cycle arrest.Mutations in LKB1 gene are associated with Peutz Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. LKB1 functions as a master upstream protein kinase, regulating AMPK related kinases as well as AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.