Western blot analysis of Phospho-LRRK2 pSer935 in whole cell extracts of U2OS expressing full length LRRK2-GFP using a Phospho-LRRK2 pSer935 recombinant rabbit oligoclonal antibody (Product # 710097) at a dilution of 2.5ug/ml. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Results show a band at ~290kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acids 930–940 of human LRRK2|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
LRRK2 belongs to the leucine-rich repeat kinase family commonly known as Dardarin. The LRRK2 gene encodes for a protein with an ankyrin-repeat region, a leucine-rich repeat region, a kinase domain, a RAS domain, a GTPase-domain, a MLK-like domain, and a WD40 domain. Proteins with leucine-rich regions play important roles in activities that require protein-protein interactions, such as transmitting signals or helping to assemble the cell's structural framework. Phosphorylation of LRRK2 at multiple sites including serine 910 and serine 935 regulates LRRK2 mediated cellular activities by affecting binding of dimeric 14-3-3 protein. Phosphorylation at Ser910, found to be constitutive in various tissues, is caused by protein kinase A. In humans, he gene is present on the q arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.