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          • Primary Antibodies ›
          • LYN/LCK/HCK/BLK Antibodies

          Invitrogen

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          Advanced Verification

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          • Antibody Testing Data (5)
          • Advanced Verification (3)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody (MA562902) in ICC/IF

          Immunofluorescence analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) using Ramos cells serum starved for 16 hours then treated with 12μg/mL human IgM (diluted in serum free medium) for 2 minutes. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          Product Details

          MA562902

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Artificial Control / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          PSH09-43

          Immunogen

          Synthetic phospho-peptide corresponding to residues surrounding Tyr394 of human LCK.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Positive Control: COLO205 cell lysate, TT cell lysate, Ramos serum starved for 16 hours cell lysate, Ramos serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Ramos cells serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes.

          Tissue Specificity: Tissue enhanced (bone marrow, lymphoid tissue).

          Subcellular Location: Cell membrane, Cytoplasm, cytosol.

          Sequence Similarities: 100% Mouse/Rat.

          Predicted band size: 58 kDa

          Target Information

          The LYN, LCK, HCK, and BLK genes encode various members of the Src family of non-receptor tyrosine kinases, each playing crucial roles in cellular signaling pathways that regulate immune function, cell proliferation, and differentiation. LYN is involved in B-cell receptor signaling, influencing cell proliferation, differentiation, apoptosis, migration, metabolism, and roles in inflammation and cancer, particularly within the immune system and oncogenic pathways. LCK is primarily expressed in T-cells and is crucial for T-cell development, function, and signaling, impacting cytokine production and immune responses. HCK, primarily found in hematopoietic cells, influences myeloid cell function and is implicated in oncogenic signaling pathways, potentially contributing to cancer progression via altered immune responses. BLK participates mainly in B-cell receptor signaling, impacting B-cell development and activation, with implications for immune cell signaling and potential roles in autoimmune diseases. Each of these kinases is a potential therapeutic target, given their roles in various pathophysiological conditions and their impact on immune cell signaling and cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: B cell/myeloid kinase; B lymphocyte kinase; B lymphoid tyrosine kinase; B-cell/myeloid kinase; BLK nonreceptor tyrosine kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; hemopoietic cell tyrosine kinase; Lck/Yes-related novel protein tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; lyn protein non-receptor kinase; p53Lyn; p55-Blk; p56(LSTRA) protein-tyrosine kinase; p56-HCK; p56-HCK/p59-HCK; p56-LCK; p56Hck; p56Lyn; p59-HCK/p60-HCK; p59Hck; p61Hck; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Blk; Tyrosine-protein kinase HCK; Tyrosine-protein kinase Lck; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

          View more View less

          Gene Aliases: AA407514; AI849071; BLK; Bmk; HCK; Hck-2; Hck-3; Hctk; IMD22; JTK8; JTK9; LCK; Lck1; Lcktkr; LSK; Lsk-t; Lskt; LYN; MODY11; p53Lyn; p56; p56Hck; p56Lck; p56Lyn; p59Hck; p61Hck; pp58lck; YT16

          View more View less

          UniProt ID: (Human) P51451, (Mouse) P16277, (Human) P08631, (Rat) P50545, (Mouse) P08103, (Human) P06239, (Human) P07948, (Rat) Q01621, (Mouse) P06240, (Mouse) P25911, (Rat) Q07014

          View more View less

          Entrez Gene ID: (Human) 640, (Mouse) 12143, (Rat) 364403, (Human) 3055, (Rat) 25734, (Mouse) 15162, (Human) 3932, (Human) 4067, (Rat) 313050, (Mouse) 16818, (Mouse) 17096, (Rat) 81515

          View more View less

          Function(s)
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups molecular_function glycoprotein binding protein serine/threonine phosphatase activity protein C-terminus binding protein kinase binding protein phosphatase binding SH2 domain binding CD4 receptor binding CD8 receptor binding identical protein binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity ATPase binding receptor signaling protein tyrosine kinase activity platelet-derived growth factor receptor binding integrin binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding antigen binding protein complex binding erythropoietin receptor binding enzyme binding kinase modulator
          Process(es)
          cytoskeleton organization transmembrane receptor protein tyrosine kinase signaling pathway cell differentiation positive regulation of insulin secretion intracellular signal transduction peptidyl-tyrosine autophosphorylation regulation of cell proliferation negative regulation of apoptotic process innate immune response regulation of phagocytosis B cell receptor signaling pathway regulation of podosome assembly protein phosphorylation phosphorylation peptidyl-tyrosine phosphorylation T cell differentiation cellular response to peptide hormone stimulus biological_process leukocyte migration involved in immune response innate immune response-activating signal transduction inflammatory response cell adhesion integrin-mediated signaling pathway mesoderm development positive regulation of cell proliferation regulation of cell shape viral process cytokine-mediated signaling pathway positive regulation of actin filament polymerization lipopolysaccharide-mediated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis leukocyte degranulation respiratory burst after phagocytosis protein autophosphorylation regulation of defense response to virus by virus regulation of inflammatory response regulation of sequence-specific DNA binding transcription factor activity interferon-gamma-mediated signaling pathway positive regulation of actin cytoskeleton reorganization exocytosis phagocytosis defense response to Gram-positive bacterium immune system process protein dephosphorylation cellular zinc ion homeostasis activation of cysteine-type endopeptidase activity involved in apoptotic process regulation of phosphatidylinositol 3-kinase signaling hemopoiesis platelet activation T cell costimulation response to drug phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling T cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of T cell activation leukocyte migration release of sequestered calcium ion into cytosol regulation of lymphocyte activation positive regulation of intrinsic apoptotic signaling pathway B cell homeostasis regulation of cytokine production regulation of protein phosphorylation negative regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway adaptive immune response Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process cellular response to DNA damage stimulus response to sterol depletion signal transduction central nervous system development blood coagulation negative regulation of cell proliferation response to toxic substance response to hormone response to carbohydrate positive regulation of neuron projection development oligodendrocyte development response to organic cyclic compound signal transduction by protein phosphorylation erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development cellular response to extracellular stimulus response to insulin regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway cellular response to heat Fc-epsilon receptor signaling pathway positive regulation of tyrosine phosphorylation of STAT protein response to amino acid regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity regulation of erythrocyte differentiation ephrin receptor signaling pathway response to axon injury cytokine secretion regulation of cytokine secretion negative regulation of immune response regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process apoptotic process cell surface receptor signaling pathway aging response to mechanical stimulus response to metal ion response to zinc ion positive regulation of gene expression dephosphorylation positive regulation of tyrosine phosphorylation of Stat5 protein response to hydrogen peroxide regulation of apoptotic process positive regulation of gamma-delta T cell differentiation regulation of T cell receptor signaling pathway positive regulation of uterine smooth muscle contraction positive regulation of phosphorylation hemoglobin biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation chemotaxis cell migration response to peptide hormone
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