Western blot analysis of Phospho-Lck pSer59 in Lane 1: extracts from Jurkat cells treated with IFN (2500U/ML, 30mins) and Lane 2: extracts from Jurkat cells treated with IFN (2500U/ML, 30mins) plus phosphopeptide using a Phospho-Lck pSer59 polyclonal anti
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human LCK around the phosphorylation site of Ser59 (P-A-SP-P-L)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.