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Lck Protein Schematic–Lck is a 56 kDa, membrane-associated, non-receptor tyrosine kinase belonging to the Src family that plays a critical role in T-cell antigen receptor (TCR) signaling, inducing T-lymphocyte development, activation, proliferation and survival. Lck has been implicated in various diseases including cancer development and metastasis, viral infections relating to HIV, heart disease, and osteoclast-mediated bone resorption. Lck is regulated by phosphorylation on multiple residues including Ser-194 in the SH2 domain, Tyr-394 (autophosphorylation site) within the activation loop of the catalytic domain, and Tyr-505 (inhibitory site) near the C-terminus. TCR requires Lck for thymocyte differentiation and activation. Additionally, Lck is implicated in JAK and STAT signaling, which are constitutively activated in cells overexpressing Lck.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide from human Lck containing tyrosine 505 (based on Swiss Protein database, accession number P06239). The sequence is conserved in mouse.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EC 188.8.131.52; kinase Lck; LCK; leukocyte C-terminal Src kinase; LSK; LSK-T; lymphocyte cell-specific protein-tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; P56-LCK; proto-oncogene tyrosine-protein kinase LCK; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck
IMD22; LCK; LSK; p56lck; pp58lck; YT16