|Tested species reactivity||Chicken, Fish, Human, Mouse, Reptile, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide made to a C-terminal portion of rat MAP1B.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||1:1000-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS, pH 7.2.
phospho MAP1B antibody, also known as SuperBUGS, is useful for detecting phosphorylated MAP1B at Thr1265. MAP1B is a developmentally regulated microtubule associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. It is phosphorylated by GSK3 beta at Ser1260 and Thr1265. MAP1B plays an important role in neurite growth, growth-cone function and neuronal migration. MAP1B is also implicated in a number of neurological disorders including giant axonal neuropathy and Fragile X syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.