|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to aa residues surrounding phospho-Thr202 and Tyr204 of ERK/MAPK|
|Storage buffer||HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide; immunolabeling can be completely eliminated by treatment with lambda-phosphatase. A suggested positive control for this product is Jurkat or rat brain lysate.
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.