Western blot analysis of Phospho-MAPKAPK-2 pThr222 in extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+) using Phospho-MAPKAPK-2 pThr222 monoclonal antibody (Product # MA5-14894) (upper) or a MAPKAPK-2 polyclonal antibody (lower).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr222 of human MAPKAPK-2|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BMP-2 induction of Dlx3 expression is mediated by p38/Smad5 signaling pathway in osteoblastic MC3T3-E1 cells.
MA5-14894 was used in western blot to study the role of p38/Smad5 signaling in the mechanism by which BMP-2 induces the expression of Dlx3 in osteoblasts
|Yang G,Yuan G,Li X,Liu P,Chen Z,Fan M||Journal of cellular physiology (229:943)||2014|