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Invitrogen
Phospho-MARCKS (Ser152, Ser156) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-MARCKS (Ser152, Ser156) Antibody (PA1-4629) in WB
Western blot of MARCKS in rat brain lysate showing specific immunolabeling of a band at ~87 kDa corresponding to Phospho-MARCKS (Ser152, Ser156) polyclonal antibody (Product # PA1-4629) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-4629
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken, human, mouse, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots of rat brain homogenates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Target Information
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 80K protein; FLJ14368; FLJ90045; MARCS; Myristoylated alanine-rich protein kinase C substrate; protein kinase C substrate 80 kDa protein
Gene Aliases: KINC; Macs; PKCSL
UniProt ID: (Mouse) P26645
Entrez Gene ID: (Mouse) 17118, (Rat) 25603
intracellular protein transport
apoptotic process
mitochondrion organization
actin filament organization
central nervous system development
regulation of cell shape
positive regulation of neuron projection development
neural tube development
neurogenesis
cell-substrate adhesion
response to endoplasmic reticulum stress
positive regulation of insulin receptor signaling pathway
actin filament bundle assembly
actin crosslink formation
positive regulation of dendritic spine morphogenesis
regulation of modification of postsynaptic actin cytoskeleton
ventricular cardiac muscle tissue development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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