Western blot analysis of Phospho-MARCKS pSer163 using Phospho-MARCKS pSer163 polyclonal antibody (Product # PA5-36852) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with PMA, Lane 2: sp2/0 cell lysate treated with PMA, Lane 3: PC12 cell lysate treated with PMA.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 163|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 32, 75 and 87 kDa.
Purity is >95% by SDS-PAGE.
Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC alpha and PKC epsilon, but not PKC delta, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.