Western blot analysis of Phospho-MARK Family in extracts from 293 cells transfected with either wild-type or threonine to alanine mutations at the respective phosphorylation sites of the MARK family members using Phospho-MARK Family polyclonal antibody (Product # PA5-17495). Transfected constructs contain a GST and HA tag. Transfection efficiency monitored with an HA antibody.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with MARK4.
It is not recommended to aliquot this antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.
PA5-17495 was used in western blot to show that NMDA receptors activate MARK/Par1 via protein kinase A in primary hippocampal neurons
|Bernard LP,Zhang H||PloS one (10:null)||2015|
MARK4 and MARK3 associate with early tau phosphorylation in Alzheimer's disease granulovacuolar degeneration bodies.
PA5-17495 was used in immunohistochemistry to study the significance for Alzheimer's disease of the presence in granulovacuolar degradation bodies of MARK-3/MARK-4 and Ser262 phosphorylated tau
|Lund H,Gustafsson E,Svensson A,Nilsson M,Berg M,Sunnemark D,von Euler G||Acta neuropathologica communications (2:null)||2014|