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Phospho-MCM4 (Ser54) Antibody (PA5-36859) in WB
Western blot analysis of Phospho-MCM4 (Ser54) in Lane 1: LO2 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: sp2/0 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-MCM4 (Ser54) polyclonal antibody (Product # PA5-36859) at a dilution of 1:500.
Product Details
PA5-36859
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of Serine 54
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2553785
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 90 kDa.^M
^M
Purity is >95% by SDS-PAGE.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC21 homolog; DNA replication licensing factor MCM4; homolog of S. pombe cell devision cycle 21; mini chromosome maintenance deficient 4 homolog; minichromosome maintenance deficient 4; minichromosome maintenance deficient 4 homolog; P1-CDC21
Gene Aliases: 19G; AI325074; AU045576; CDC21; CDC54; hCdc21; mcdc21; MCM4; Mcmd4; mKIAA4003; NKCD; NKGCD; P1-CDC21
UniProt ID: (Human) P33991, (Mouse) P49717
Entrez Gene ID: (Human) 4173, (Rat) 29728, (Mouse) 17217
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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