Immunohistochemical analysis of Phospho-MDM2 pSer185 in formalin-fixed, paraffin-embedded human prostate tissue using a Phospho-MDM2 pSer185 polyclonal antibody (Product # PA1-31438) at a dilution of 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide HRKRRRSLSFDPSLGLCEL, corresponding to amino acids 177-198 of Mouse MDM2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31438 detects MDM2 from human and mouse samples.
PA1-31438 has been successfully used IP, IHC-P, ELISA, and Western blot applications.
The PA1-31438 immunogen is: Synthetic peptide: HRKRRRSLSFDPSLGLCEL, corresponding to amino acids 177-198 of Mouse MDM2.
Clone SMP14 recognizes the 90kDa MDM2 protein. MDM2 forms a complex with p53 which leads to inhibition of p53 function. MDM2 has also been shown to stimulate cellular proliferation via induction of transcriptional regulators including E2F1 and DP1. MDM
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.