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Immunofluorescent analysis of Phospho-MEK1 + MEK2 pSer221 in mouse embryonic fibroblasts using a Phospho-MEK1 + MEK2 pSer221 polyclonal antibody (Product # PA1-26646) showing cell division. Blue represents chromosomes in anaphase of mitotic cell division. Green shows mitotic spindle expressing phosphorylated MEK1/2.
|Tested species reactivity||Human, Mouse, Primate, Rat, Clawed frog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human MEK 1 + 2 containing serine 222. The sequence is conserved in many species including mous|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26646 detects MEK1 + MEK2 (phospho S222) from human, mouse, and rat samples. PA1-26646 is expected to cross react with chicken, chimpanzee, and Xenopus laevis due to sequence homology.
PA1-26646 has been successfully used in Western blot procedures.
The PA1-26646 immunogen is a synthetic phosphopeptide corresponding to a portion of human MEK 1 + 2 containing serine 222. The sequence is conserved in many species including mouse, rat, chimp, chicken, frog, and others.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
dual specificity mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; MAP kinase kinase 1; MAP kinase kinase 2; MAP kinase kinase or Erk Kinase, Dual specificity mitogen-activated protein kinase kinase, involved in ras mediated vulval induction, LEThal LET-537 (42.8 kD) (mek-2); MAP kinase/Erk kinase; MAP kinase/Erk kinase 1; MAP2K1; Map2k2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK1; MEK2; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen-activated kinase 1; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2, p45; MKK2; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen activated, kinase 2, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
AA589381; CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; mek-2; MEK1; MEK2; MEKK1; MK2; MKK1; MKK2; PRKMK1; PRKMK2