Invitrogen
Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated. View details.
Catalog #:44-460G
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| TESTED APPLICATIONS | DILUTION |
|---|---|
| Immunocytochemistry (ICC) | 1:250 |
| Immunofluorescence (IF) | 1:250 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20-1:200 |
| Western Blot (WB) | 1:500-1:2000 |
| PUBLISHED APPLICATIONS | |
|---|---|
| Western Blot (WB) | See 2 publications below |
| Tested Species reactivity | Human, Mouse, Rat |
| Published species reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK1 that contains serine 298. The sequence is conserved in many species including mouse, rat, chimp, hamster, and rabbit. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C |
| RRID | AB_2533657 |
MAP2K1 is a dual specificity mitogen-activated protein kinase (MAPK) kinase. It is an essential component of the MAP kinase pathway and stimulates the enzymatic activity of MAP kinases in response to various signals. Growth factors, cytokines, and proto-oncogenes transduce their signals through the activation of RAS, which leads to activation of the serine/threonine kinase RAF, and then to activation of MAPK kinase. MAP2K1 is activated by the Raf family member p74raf-1, through phosphorylation of serine residues at positions 217 and 221 in the activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MP2K1; PRKMK1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1
UniProt ID: (Human) Q02750, (Rat) Q01986, (Mouse) P31938
Entrez Gene ID: (Human) 5604, (Rat) 170851, (Mouse) 26395
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