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Phospho-MKP-1 (Ser359) Antibody (PA5-105159) in IHC (P)
Immunohistochemistry analysis of Phospho-MKP-1 (Ser359) in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-MKP-1 (Ser359) polyclonal antibody (Product # PA5-105159) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-105159
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser359.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816632
Product Specific Information
Antibody detects endogenous levels of DUSP1/MKP1 only when phosphorylated at Ser359.
Target Information
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase-1; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; serine/threonine specific protein phosphatase
Gene Aliases: 3CH134; CL100; DUSP1; erp; HVH1; MKP-1; MKP1; PTPN10; Ptpn16; U19515; VH1
UniProt ID: (Human) P28562, (Mouse) P28563
Entrez Gene ID: (Human) 1843, (Mouse) 19252
non-membrane spanning protein tyrosine phosphatase activity
protein binding
protein tyrosine/threonine phosphatase activity
MAP kinase tyrosine/serine/threonine phosphatase activity
growth factor binding
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
protein tyrosine/serine/threonine phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
inactivation of MAPK activity
endoderm formation
response to oxidative stress
response to light stimulus
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
peptidyl-threonine dephosphorylation
response to hydrogen peroxide
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
negative regulation of ERK1 and ERK2 cascade
mitotic cell cycle arrest
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of DNA biosynthetic process
protein dephosphorylation
cell cycle
dephosphorylation
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