Extracts of HeLa cells spiked with chicken gizzard myosin II left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with the MLC [pS19] Polyclonal Antibody, Rabbit (Cat. No. 44260G).
|Tested species reactivity||Chicken, Human|
|Published species reactivity||Rat, Bovine, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of MLC that contains serine 19 (serine 20 including the initiating methionine).|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border.
44-260G was used in western blot to study inhibition of Rac1 trafficking to the cell border by RhoB control of the endothelial barrier recovery
|Marcos-Ramiro B,García-Weber D,Barroso S,Feito J,Ortega MC,Cernuda-Morollón E,Reglero-Real N,Fernández-Martín L,Durán MC,Alonso MA,Correas I,Cox S,Ridley AJ,Millán J||The Journal of cell biology (213:385)||2016|
A novel function of nuclear nonmuscle myosin regulatory light chain in promotion of xanthine oxidase transcription after myocardial ischemia/reperfusion.
44-260G was used in ChIP assay, immunocytochemistry, and western blot to study the involvement of nuclear nonmuscle myosin regulatory light chain in response to myocardial ischemia/reperfusion
|Zhang YS,Liu B,Luo XJ,Zhang JJ,Li NS,Ma QL,Jiang JL,Li YJ,Li Q,Peng J||Free radical biology and medicine (83:115)||2015|
|p21-activated kinase regulates endothelial permeability through modulation of contractility.||Stockton RA,Schaefer E,Schwartz MA||The Journal of biological chemistry (279:46621)||2004|