|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18 (A-T-SP-N-V)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MLC-2; MLC-2A; MLC20; MRLC2; MYLC2B; myosin light chain, regulatory B; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin regulatory light chain-B; Myosin RLC-B; myosin, light chain 12B, regulatory; SHUJUN-1
Gene Aliases: 1500001M02Rik; C77744; MLC-B; MRLC2; Mrlcb; MYL12B; MYLC2B; RLC-B
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