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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Ser376 (G-Y-SP-F-V)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
90 kDa ribosomal protein S6 kinase; 90 kDa ribosomal protein S6 kinase 5; kinase MSK1; KS6A5; MSPK1; nuclear mitogen- and stress-activated protein kinase 1; Nuclear mitogen- and stress-activated protein kinase-1; polypeptide 5; polypeptide V; ribosomal protein S6 kinase; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RLSK; RPS6KA5; RSK-like protein kinase; RSKL; S6K-alpha-5
3110005L17Rik; 6330404E13Rik; AI854034; MSK1; MSPK1; RLPK; RPS6KA5
annexin calcium-binding protein calmodulin cytoskeletal protein intracellular calcium-sensing protein kinase microtubule family cytoskeletal protein non-motor microtubule binding protein non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase