|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of threonine 568 (M-Q-T(p)-P-C) derived from Human MSK2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with mouse based on sequence homology.
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 90 kDa ribosomal protein S6 kinase 4; EC 188.8.131.52; kinase MSK2; KS6A4; mitogen- and stress-activated protein kinase 2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSK-B; RSKB; S6K-alpha-4; Similar to ribosomal protein S6 kinase, 90kD, polypeptide 4
Gene Aliases: MSK2; RPS6KA4; RSK-B; S6K-alpha-4
UniProt ID: (Human) O75676
Entrez Gene ID: (Human) 8986
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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