|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human Met around the phosphorylation site of Tyrosine 1234|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-Met; EC 18.104.22.168; hepatocyte growth factor receptor; Hepatocyte growth factor receptor precursor; HGF receptor; HGF-SF receptor; HGF/SF receptor; kinase EC 22.214.171.124; Met proto- oncogene tyros; Met proto- oncogene tyrosine kinase; met proto-oncogene tyrosine kinase; proto-oncogene c-Met; scatter factor receptor; SF receptor; tyrosine-protein kinase Met
Gene Aliases: AI838057; AUTS9; c-Met; HGF; HGFR; Par4; RCCP2
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