Western blot analysis of Phospho-Mucin 1/MUC-1 pTyr1229 using Phospho-Mucin 1/MUC-1 pTyr1229 polyclonal antibody (Product # PA5-36860) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with PMA (100nM, 15min), Lane 2: NIH-3T3 cell lysate treated with PMA (100nM, 15min), Lane 3: H9C2 cell lysate treated with PMA (100nM, 15min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Mucin 1 around the phosphorylation site of Tyrosine 1229|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 122 and 170 kDa.
Purity is >95% by SDS-PAGE.
MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.