Immunofluorescent analysis of Phospho-Myosin Light Chain 2 pThr18/Ser19 in HeLa cells using a Phospho-Myosin Light Chain 2 pThr18/Ser19 polyclonal antibody (Product # PA5-17727) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr18/Ser19 of human myosin light chain 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the cardiac isoform of myosin light chain 2.
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rnd3 regulates lung cancer cell proliferation through notch signaling.
PA5-17727 was used in western blot to explore the role of Rnd3 in non-small cell lung cancer
|Tang Y,Hu C,Yang H,Cao L,Li Y,Deng P,Huang L||PloS one (9:null)||2014|
Rnd3 haploinsufficient mice are predisposed to hemodynamic stress and develop apoptotic cardiomyopathy with heart failure.
PA5-17727 was used in western blot to study heart failure in Rnd3(+/-) haploinsufficient mice
|Yue X,Yang X,Lin X,Yang T,Yi X,Dai Y,Guo J,Li T,Shi J,Wei L,Fan GC,Chen C,Chang J||Cell death and disease (5:null)||2014|
New insights into myosin phosphorylation during cyclic nucleotide-mediated smooth muscle relaxation.
PA5-17727 was used in western blot to study the effects of cyclic nucleotide-mediated smooth muscle relaxation on the phosphorylation patterns of myosin regulatory light chains
|Puetz S,Schroeter MM,Piechura H,Reimann L,Hunger MS,Lubomirov LT,Metzler D,Warscheid B,Pfitzer G||Journal of muscle research and cell motility (33:471)||2012|