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Immunohistochemical analysis of Phospho-Myosin, Light chain pSer20 in a variety of tissues including multi-human, multi-brain and multi-cancer slides using a Phospho-Myosin, Light chain pSer20 polyclonal antibody (Product # PA1-26470) at a dilution of 2.5 µg/ml. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed, paraffin-embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues K(12) K R P Q R A T (pS) N V F A M F D(27) of the human Myosin Light Chain protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:5000 - 1:20,000|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26470 detects Myosin light chain (phospho S20) from human samples. PA1-26470 is expected to cross react with all mammals and birds due to 100% sequence homology.
PA1-26470 has been successfully used in Western blot, IF, ICC, IHC (P) and ELISA procedures.
The PA1-26470 immunogen is a synthetic phosphopeptide corresponding to residues K(12) K R P Q R A T (pS) N V F A M F D(27) of the human Myosin Light Chain protein.
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A1 catalytic; A2 catalytic; MLC1/MLC3; MLC1F; MLC1F/MLC3F; MLC3F; myosin light chain 1/3, skeletal muscle isoform; myosin light chain A1/A2; myosin light chain alkali 1/2; myosin light chain, alkali, fast skeletal muscle; myosin, light chain 1, alkali; skeletal, fast; myosin, light polypeptide 1, alkali; atrial, embryonic; myosin, light polypeptide 1, alkali; skeletal, fast; myosin, light polypeptide 4, alkali; atrial, embryonic
AI325107; MLC1F; MLC3F; MYL1; Mylf