Western blot analysis of Phospho-NCF1/p47 phox pSer359 in Nocodazole-treated HeLa whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-NCF1/p47 phox pSer359 polyclonal antibody (Product # PA5-35745).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human p47 phox around the phosphorylation site of Serine 359|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.