Immunofluorescent analysis of Phospho-NF-kappaB p65 pSer536 in HeLa cells, TNF-alpha treated (20 ng/ml for 20 min), using a Phospho-NF-kappaB p65 pSer536 monoclonal antibody (Product # MA5-15160) (green). Actin filaments are labeled with a fluorescent red phalloidin.
|Tested species reactivity||Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer536 of human NFkB p65|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:1600|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the p50 subunit or other related proteins.
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle.
MA5-15160 was used in western blot to learn about the induction of inflammatory signaling in porcine skeletal muscle after twelve hours of heat stress
|Ganesan S,Reynolds C,Hollinger K,Pearce SC,Gabler NK,Baumgard LH,Rhoads RP,Selsby JT||American journal of physiology. Regulatory, integrative and comparative physiology (310:R1288)||2016|
Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo.
MA5-15160 was used in western blot to elucidate the reduction of chemotherap-enriched breast cancer stem-like cells in vivo and in vitro due to cardamonin
|Jia D,Tan Y,Liu H,Ooi S,Li L,Wright K,Bennett S,Addison CL,Wang L||Oncotarget (7:771)||2016|
ß-Catenin and NF-¿B co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.
MA5-15160 was used in western blot to investigate the role of TLR3 signaling in breast cancer stem cells
|Jia D,Yang W,Li L,Liu H,Tan Y,Ooi S,Chi L,Filion LG,Figeys D,Wang L||Cell death and differentiation (22:298)||2015|
Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats.
MA5-15160 was used in immunohistochemistry to investigate the protective effect of Coenzyme Q10 againstsodium arsenite-induced testicular injury
|Fouad AA,Al-Sultan AI,Yacoubi MT||European journal of pharmacology (655:91)||2011|