Western blot analysis of Phospho-NF2 pSer518 in 12 ul of mouse cardiac myocyte lysate either mock-treated (lanes 1, 5) or insulin treated at 10nM, 100nM and 1µM (lanes 2, 3,4) for 15 min, or CLA treated at 4nM, 20nM or 100nM (lanes 6, 7, 8) for 45 min. Samples were loaded on a 4-20% Criterion gel for SDS-PAGE and probed with a Phospho-NF2 pSer518 polyclonal antibody (Product # PA1-28383) at a dilution of 1:5000 and detected using an HRP conjugated Gt-anti-Rabbit IgG secondary antibody (color development using Amersham's substrate system).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human NF2 / Merlin phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - TDMKRL(pS)MEIEK (aa 508-530).|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28383 detects NF2 from mouse, human samples.
PA1-28383 has been successfully used in Western blot applications.
The PA1-28383 immunogen is: Human NF2 / Merlin phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - TDMKRL(pS)MEIEK (aa 508-530).
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.