Invitrogen

Phospho-NFkB p65 (Ser536) Monoclonal Antibody (T.849.2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

20 References

View all (142) NFkB p65 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-NFkB p65 (Ser536) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

Phospho-NFkB p65 (Ser536) Antibody (MA5-15160) in ICC/IF

Immunofluorescent analysis of Phospho-NF-kappaB p65 pSer536 in HeLa cells, TNF-alpha treated (20 ng/mL for 20 min), using a Phospho-NF-kappaB p65 pSer536 monoclonal antibody (Product # MA5-15160) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Product Image Gallery

Phospho-NFkB p65 (Ser536) Antibody in Western Blot (WB) — J-Lat Full Length cell lysate 40 µg was loaded on 4-15% gel and transferred to 0.45 µm PVDF Membrane. Phospho-NFkB p65 (Ser536) Monoclonal Antibody (Product # MA5-15160) was diluted at 1:500 in 1xTBST buffer with 5% BSA and incubated overnight at 4C. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at 21C.

Phospho-NFkB p65 (Ser536) Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Phospho-NF-kappaB p65 pSer536 in untreated (blue) or TNF-alpha-treated (green, 20 ng/mL for 10 minutes) HeLa cells using a Phospho-NF-kappaB p65 pSer536 monoclonal antibody (Product # MA5-15160) compared to a nonspecific negative control antibody (red).

Phospho-NFkB p65 (Ser536) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 4 Effects of capsaicin and LPS treatment on the phosphorylation of NF-kappaB p65 in LPS-challenged IPEC-J2 cells. The level of phosphorylated NF-kappaB p65 was evaluated using Immunofluorescence staining (A) and Western blotting (B) , respectively. For Immunofluorescence staining, IPEC-J2 cells were seeded into coverslips at a density of 1x10 5 /well and cultured for 2 weeks. After being pretreated with capsaicin (100 muM), cells were stimulated with LPS. Cells then were then fixed for phosphorylated NF-kappaB p65 staining as described in the Materials and Methods. As for Western blot, IPEC-J2 cells were cultured in 6-well plates. Cells were firstly pre-treated with capsaicin (100 muM) for 2 h and then stimulated with LPS (10 mug/mL) for 6 h. The protein was extracted and the total NF-kappaB p65, as well as phosphorylation of NF-kappaB p65 in LPS-challenged IPEC-J2 cells were detected as described in the Materials and Methods. Values are presented as mean +- SEM. Different letters on bars (a, b) indicate significant differences, P < 0.05.

Phospho-NFkB p65 (Ser536) Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western Blot of Phospho-NFkB p65 (Ser536) using Anti-Phospho-NFkB p65 (Ser536) Monoclonal Antibody (Product # MA5-15160), shows increased expression of Phospho-NFkB p65 (Ser536) in HeLa cells upon TNF alpha treatment. {TM}

Product Details

MA5-15160

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:800-1:3200

View 2 publications 2 publications

Flow Cytometry (Flow)

1:800-1:3200

View 2 publications 2 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Pig, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

T.849.2

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer536 of human NFkB p65

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

57 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10983078

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the p50 subunit or other related proteins.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Remifentanil modulates the TLR4‑mediated MMP‑9/TIMP1 balance and NF‑κB/STAT3 signaling in LPS‑induced A549 cells.

Experimental and therapeutic medicine

Chang J,Zhang W

MA5-15160 was used in Western Blotting to show that remifentanil downregulated TLR4 to reduce MMP-9/TIMP1 imbalance to inhibit inflammatory dysfunction in LPS-treated A549 cells, by regulating NF-κB/STAT3 signaling.

Wed Feb 01 00:00:00 EST 2023

B-cell antigen receptor expression and phosphatidylinositol 3-kinase signaling regulate genesis and maintenance of mouse chronic lymphocytic leukemia.

Haematologica

Schmid VK,Khadour A,Ahmed N,Brandl C,Nitschke L,Rajewsky K,Jumaa H,Hobeika E

MA5-15160 was used in Flow Cytometry to demonstrate the pivotal role for B cell antigen receptor signalling in chronic lymphocytic leukemia (CLL) development and further understand the molecular mechanisms underlying the genesis of CLL for the development of new treatment strategies.

Mon Aug 01 00:00:00 EDT 2022

Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.

Nature communications

Chandana M,Anand A,Ghosh S,Das R,Beura S,Jena S,Suryawanshi AR,Padmanaban G,Nagaraj VA

MA5-15160 was used in Western Blotting to show heme synthesized in asexual stages promotes cerebral pathogenesis by enhancing hemozoin formation.

Tue Jul 12 00:00:00 EDT 2022

Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy.

Nature communications

Liu H,Stepicheva NA,Ghosh S,Shang P,Chowdhury O,Daley RA,Yazdankhah M,Gupta U,Hose SL,Valapala M,Fitting CS,Strizhakova A,Shan Y,Feenstra D,Sahel JA,Jayagopal A,Handa JT,Zigler JS,Fort PE,Sodhi A,Sinha D

MA5-15160 was used in Western Blotting to propose that targeting Akt1 activity in the RPE may be a novel therapy for treating DR.

Thu Oct 13 00:00:00 EDT 2022

Haloperidol Instigates Endometrial Carcinogenesis and Cancer Progression by the NF-κB/CSF-1 Signaling Cascade.

Cancers

Chiang JY,Lei FJ,Chang HJ,Wei ST,Wang CC,Huang YC,Wang HL,Chuang CF,Hu SY,Hsieh CH

MA5-15160 was used in Western Blotting to show that chronic haloperidol treatment in clinically relevant doses induced endometrial hyperplasia in normal mice and promoted tumor growth and malignancy in mice with orthotopic endometrial cancer.

Thu Jun 23 00:00:00 EDT 2022

Fetuin-A secretion from β-cells leads to accumulation of macrophages in islets, aggravates inflammation and impairs insulin secretion.

Journal of cell science

Mukhuty A,Fouzder C,Kundu R

MA5-15160 was used in Western Blotting to support an association of fetuin-A with islet inflammation and β-cell dysfunction, owing to its role as a key chemoattractant and macrophage polarizing factor.

Mon Nov 01 00:00:00 EDT 2021

Capsaicin Attenuates Lipopolysaccharide-Induced Inflammation and Barrier Dysfunction in Intestinal Porcine Epithelial Cell Line-J2.

Frontiers in physiology

Zhao X,Dong B,Friesen M,Liu S,Zhu C,Yang C

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

Capsaicin Attenuates Lipopolysaccharide-Induced Inflammation and Barrier Dysfunction in Intestinal Porcine Epithelial Cell Line-J2.

Frontiers in physiology

Zhao X,Dong B,Friesen M,Liu S,Zhu C,Yang C

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Communications biology

Ghosh S,Liu H,Yazdankhah M,Stepicheva N,Shang P,Vaidya T,Hose S,Gupta U,Calderon MJ,Hu MW,Nair AP,Weiss J,Fitting CS,Bhutto IA,Gadde SGK,Naik NK,Jaydev C,Lutty GA,Handa JT,Jayagopal A,Qian J,Sahel JA,Rajasundaram D,Sergeev Y,Zigler JS,Sethu S,Watkins S,Ghosh A,Sinha D

MA5-15160 was used in Western Blotting to identify βA1-crystallin as a regulator of PTP1B; loss of this regulation may be a new mechanism by which astrocytes contribute to DR.

Wed Feb 24 00:00:00 EST 2021

Activation of vagovagal reflex prevents hepatic ischaemia-reperfusion-induced lung injury via anti-inflammatory and antioxidant effects.

Experimental physiology

Deng J,Jiang Y,Wang M,Shao L,Deng C

MA5-15160 was used in Western Blotting to suggest that VNS might protect patients from lung injury induced by hepatic I/R related to various circumstances.

Mon Nov 01 00:00:00 EDT 2021

Roxatidine inhibits fibrosis by inhibiting NF‑κB and MAPK signaling in macrophages sensing breast implant surface materials.

Molecular medicine reports

Ji L,Wang T,Tian L,Song H,Gao M

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.

International journal of molecular sciences

Lyu Q,Wawrzyniuk M,Rutten VPMG,van Eden W,Sijts AJAM,Broere F

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Western Blot

Fri Sep 04 00:00:00 EDT 2020

Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.

International journal of molecular sciences

Lyu Q,Wawrzyniuk M,Rutten VPMG,van Eden W,Sijts AJAM,Broere F

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Western Blot

Fri Sep 04 00:00:00 EDT 2020

ECRG4 Represses Cell Proliferation and Invasiveness via NFIC/OGN/NF-κB Signaling Pathway in Bladder Cancer.

Frontiers in genetics

Liang X,Gao J,Wang Q,Hou S,Wu C

MA5-15160 was used in Western Blotting to provide evidence that both ECRG4 and OGN function as tumor repressors and that overexpression of ECRG4 inhibits the NF-κB signaling pathway by promoting NFIC/OGN signaling in BCa cells.

Mon Sep 28 00:00:00 EDT 2020

Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells.

Nature communications

Panagiotakopoulou V,Ivanyuk D,De Cicco S,Haq W,Arsić A,Yu C,Messelodi D,Oldrati M,Schöndorf DC,Perez MJ,Cassatella RP,Jakobi M,Schneiderhan-Marra N,Gasser T,Nikić-Spiegel I,Deleidi M

MA5-15160 was used in Immunocytochemistry-immunoflourescence to employ dopaminergic neurons and microglia differentiated from patient-derived induced pluripotent stem cells carrying LRRK2 G2019S.

Wed Oct 14 00:00:00 EDT 2020

IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.

Cancer research

Gaggianesi M,Turdo A,Chinnici A,Lipari E,Apuzzo T,Benfante A,Sperduti I,Di Franco S,Meraviglia S,Lo Presti E,Dieli F,Caputo V,Militello G,Vieni S,Stassi G,Todaro M

MA515160 was used in western blot to find a role for IL-4 in promoting breast cancer aggressiveness

Thu Jun 15 00:00:00 EDT 2017

An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells.

Cell death & disease

Jia D,Li L,Andrew S,Allan D,Li X,Lee J,Ji G,Yao Z,Gadde S,Figeys D,Wang L

MA5-15160 was used in Western Blotting to provide rovides new insights into the communication between autocrine niches and signaling pathways in the development of chemotherapy resistance and CSCs and also offers a tangible approach in breast cancer treatment.

Thu Jul 13 00:00:00 EDT 2017

Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle.

American journal of physiology. Regulatory, integrative and comparative physiology

Ganesan S,Reynolds C,Hollinger K,Pearce SC,Gabler NK,Baumgard LH,Rhoads RP,Selsby JT

MA5-15160 was used in western blot to learn about the induction of inflammatory signaling in porcine skeletal muscle after twelve hours of heat stress

Wed Jun 01 00:00:00 EDT 2016

Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo.

Oncotarget

Jia D,Tan Y,Liu H,Ooi S,Li L,Wright K,Bennett S,Addison CL,Wang L

MA5-15160 was used in western blot to elucidate the reduction of chemotherap-enriched breast cancer stem-like cells in vivo and in vitro due to cardamonin

Tue Jan 05 00:00:00 EST 2016

β-Catenin and NF-κB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.

Cell death and differentiation

Jia D,Yang W,Li L,Liu H,Tan Y,Ooi S,Chi L,Filion LG,Figeys D,Wang L

Published figure using Phospho-NFkB p65 (Ser536) monoclonal antibody (Product # MA5-15160) in Western Blot

Sun Feb 01 00:00:00 EST 2015

Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats.

European journal of pharmacology

Fouad AA,Al-Sultan AI,Yacoubi MT

MA5-15160 was used in immunohistochemistry to investigate the protective effect of Coenzyme Q10 againstsodium arsenite-induced testicular injury

Fri Mar 25 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65

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Gene Aliases: I79_021812; NFkB; NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Pig) 100135665, (Mouse) 19697, (Chinese hamster) 100763542, (Rat) 309165

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Molecular Function: Rel homology transcription factor

Function(s)

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding

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Phospho-NFkB p65 (Ser536) Monoclonal Antibody (T.849.2)

Phospho-NFkB p65 (Ser536) Antibody (MA5-15160) in ICC/IF

Product Details

MA5-15160

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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