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      View (38) other NFkB p65 antibodies

      Invitrogen

      Phospho-NFkB p65 (Thr435) Polyclonal Antibody

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      Catalog #:PA5-37724

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      Product Details

      TESTED APPLICATIONS DILUTION
      Immunocytochemistry (ICC) 1:100-1:200
      Immunofluorescence (IF) 1:100-1:200
      Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:100
      Western Blot (WB) 1:500-1:1000
      Tested Species reactivity Human, Mouse, Rat
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Peptide sequence around phosphorylation site of threonine 435(E-G-T(p)-L-S) derived from Human NF-kappa-B-p65.
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.4, with 50% glycerol
      Contains 0.02% sodium azide
      Storage conditions -20°C
      RRID AB_2554332

      Product Specific Information

      A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.

      Background/Target Information

      NF-kappaB (Nuclear Factor kappa B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-kappaB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-kappaB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-kappaB family: NF-kappaB1, NF-kappaB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-kappaB is the p50/RelA heterodimer, although other forms of NF-kappaB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-kappaB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-kappaB is tightly regulated by interaction with inhibitory IkappaB proteins.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      Bioinformatics

      Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; Nuclear Factor kB p65 subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; RelA; Transcription Factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

      Gene Aliases: NFKB3; p65; RELA

      UniProt ID: (Human) Q04206, (Mouse) Q04207

      Entrez Gene ID: (Human) 5970, (Mouse) 19697

      Molecular Function: nucleic acid binding transcription factor

      Function(s) Blue Arrow Icon
      negative regulation of transcription from RNA polymerase II promoter liver development hair follicle development stimulatory C-type lectin receptor signaling pathway acetaldehyde metabolic process transcription from RNA polymerase II promoter inflammatory response cellular defense response aging positive regulation of cell proliferation organ morphogenesis response to organic substance response to UV-B positive regulation of Schwann cell differentiation cytokine-mediated signaling pathway membrane protein intracellular domain proteolysis positive regulation of chondrocyte differentiation positive regulation of type I interferon production response to muramyl dipeptide response to progesterone response to insulin response to cobalamin cellular response to hepatocyte growth factor stimulus response to muscle stretch Fc-epsilon receptor signaling pathway negative regulation of protein catabolic process response to drug negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine positive regulation of interleukin-12 biosynthetic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus negative regulation of NIK/NF-kappaB signaling positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response I-kappaB kinase/NF-kappaB signaling response to bacterium response to cytokine NIK/NF-kappaB signaling innate immune response
      Process(es) Blue Arrow Icon
      RNA polymerase II regulatory region sequence-specific DNA binding
       RNA polymerase II core promoter proximal region sequence-specific DNA binding
       RNA polymerase II distal enhancer sequence-specific DNA binding
       transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
       transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
       transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
       DNA binding
       chromatin binding
       transcription factor activity, sequence-specific DNA binding
       transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
       protein binding
       transcription factor binding
       protein kinase binding
       chromatin DNA binding
       ubiquitin protein ligase binding
       protein complex binding
       activating transcription factor binding
       phosphate ion binding
       identical protein binding
       protein homodimerization activity
       actinin binding
       histone deacetylase binding
       transcription regulatory region DNA binding
       protein heterodimerization activity
       protein N-terminus binding
       NF-kappaB binding
       repressing transcription factor binding
       ankyrin repeat binding
       enzyme binding
       sequence-specific DNA binding

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