|Tested species reactivity||Marsupial, Mouse, Non-human primate, Rabbit, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide made to a region surrounding the Serine 605 portion of the rat protein.|
|Storage buffer||sodium borate|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: PKA treated rat kidney membrane.
NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.