Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr 1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: L-Ptase). The blot is identical to the control except that it was incubated in L-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr 1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with L-Ptase.
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of rat GRIN2B conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects a ~180 kDa protein representing NMDAR NR2B-subunit protein phosphorylated at Tyr1472. It also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either lamda-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.